Featured Collection Categories
The DNA Collections is broadly separated into three main categories relating to microbial, plant, and cell-free collections.
As part of OpenPlant Initiative and the follow-on project "Engineering specialised metabolism and new cellular architectures in plants" (BB/W014173/1), the following collections were deposited into the Open DNA Collections Hub:
- The Marchantia Toolkit,
- The MinSyn Promoter Collection,
- The Triterpene Collection
The microbial collections are primarily curated from the FreeGenes and Open DNA Collections that are now stewarded by the Reclone Initiative.
It includes: the Open Enzyme Collection, the Open Reporters Collection, the Research in Diagnostics Toolkit, E.coli Protein Expression Toolkit, the Open Yeast Collection, Yeast Protein Expression Toolkit, and the B. subtilis Protein Secretion Toolkit.
The community is always looking for new suggestions and collaborations, socome to the Reclone Forum to add your ideas!
Biofoundry-assisted expression and characterization of plant proteins:
Many goals in synthetic biology, including the elucidation and refactoring of biosynthetic pathways and the engineering of regulatory circuits and networks, require knowledge of protein function. To leverage the ability of biofoundries to accelerate design–built–test–learn cycles, the Patron Lab presents a workflow for automated DNA assembly and cell-free expression of plant proteins that accelerates optimization and enables rapid screening of enzyme activity. The phytobrick-compatible Golden Gate DNA assembly toolbox developed contains plasmid acceptors for cell-free expression using Escherichia coli or wheat germ lysates as well as a set of N- and C-terminal tag parts for detection, purification and improved expression/folding.